Normal 0 0 1 196 1119 9 2 1374 11.1282 0 0 0

Odorless, non-repellent formulation with the active ingredient (S)-Hydroprene, that prevents immature bed bugs from becoming normal reproductive adults. The formulation works for you by stopping future generations of infestations. The formulation has an excellent toxicity profile and is approved for use in sensitive accounts.

The formulation works on bed bugs, as in cockroaches, by preventing the nymphs from developing into reproductively functioning adults. Electron microscopy shows that in cockroaches and in bed bugs the organs responsible for normal copulation and reproduction do not develop. In the case of male bed bugs, the paramere, or reproductive organ, fails to develop normally. As a result, successful copulation cannot occur.

In studies generated to satisfy EPA registration requirements, The formulation provided efficacy by inhibiting the development of the next generation of nymphs by 92%. In other words, nymphs exposed to The formulation treatment were unable to reproduce. Mortality was also observed in this study where 66% of the exposed nymphs failed to survive to adults. Of those surviving to adults, reproduction was significantly reduced by 92% from the untreated control population. There was an average of 3 nymphs produced vs. an average of 61 produced in the untreated control group.

Normal 0 0 1 862 4915 40 9 6035 11.1282 0 0 0

Unlike other solutions DECON PROGREEN™ solution combines
3 mechanisms of action for complete kill on contact:

• Physically denatures bacterial spore via surfactant boring holes in protein armor

• Oxidizing agents attack genetic material (DNA) within the spore

• Hydrolyzing agents attack vital bacterial spore contents and functions

Benefits

• Fast onset of action

• Air and surface environments treated

• High log-kill

• Antimicrobial inhibition

• Non-toxic once applied

• Biodegradable by-products

• Cost effective - time and flexibility

TEST RESULTS - Sierra Research Laboratories

In vitro bioassay systems are very efficient in the evaluation of the intrinsic activity of insecticide formulations against selected arthropod pest species. The ability to evaluate pest arthropods against candidate formulations under controlled laboratory conditions is critical in determining the efficacy of a product. These evaluations are designed to determine the contact kill (spray and immersion) of DECON PROGREEN™ formulation against bed bugs in the laboratory.

Bed bugs, Cimex lectularius (SRL field strain – Earl) adults and late instars. Ten (10) bed bugs (mixed age and sex) per replicate.

Efficacy evaluations were conducted by directly treating bed bugs with the test substance (~1 g) in a 10 cm Petri dish lined with mattress ticking (Test #1). A second test was conducted by applying ~1 g of the test substance into 1 oz. plastic soufflé cups with bed bugs (immersion), holding them for 1 minute and transferring them to clean untreated mattress ticking in Petri dishes (Test #2). Water controls were treated in the same manner in both tests. All test materials were applied with a hand-held trigger sprayer.

Treatments were conducted outdoors to avoid cross contamination of untreated water controls. Treatments consisted of direct spray applications to bed bugs with 5 replicates per test group and approximately 10 bed bugs of mixed age and sex placed in each replicate. Water control replicates were set-up and scored with the treated groups. Mortality was evaluated at 60 minutes, 2, 4 and 24 hours post-treatment.

Results and Discussion:

Direct application of DECON PROGREEN™ Formulation to exposed bed bugs demonstrated a high degree of mortality 60 minutes after treatment using both evaluation techniques. The direct spray application to the bed bugs on mattress ticking gave 71.7% mortality at 60-minutes and 24-hours post-treatment (Table 1) with slight recovery at 2 and 4-hours post-treatment. The immersion technique demonstrated greater mortality of bed bugs than the direct application technique in these evaluations. Bed bug mortality ranged between 96.1 and 100% (Table 1) for the 24-hour evaluation using the immersion technique. There was also slight recovery of bed bugs from 1 to 2 hours post-treatment with the immersion technique as was seen in the direct contact evaluation. Superior efficacy is most likely a function of saturation (complete coverage) of the exposed bed bugs for approximately a 1-minute exposure time.

Bed bug eggs treated with DECON PROGREEN™ Formulation darkened 24 hours post-treatment and many collapsed.  The fluid inside the eggs became yellow or brown in color 2 days post-treatment.  Some eggs appeared to be developing normally (whitish-gray in color with red eye spots) but eventually collapsed.  None of the eggs treated with DECON PROGREEN™ hatched.  Eggs in the untreated control group were whitish gray in color with red eye spots visible after 2 days and turned light tan 2-3 days before hatching.  The untreated control group demonstrated a 98.4% hatch rate.

Conclusion:

DECON PROGREEN™ Formulation applied as a direct spray application to bed bugs on mattress ticking or as a total immersion of bed bugs, demonstrated a high degree of mortality, 71.1 and 100%, respectively, twenty-four hours after treatment.

DECON PROGREEN™ Formulation applied by an air spray applicator to bed bug eggs on filter paper ≤24 hours old resulted in 100% control as an ovicidal treatment.

TEST RESULTS - Virginia Tech

DECON PROGREEN™ solution does kill bed bugs in direct spray tests.  After he conducted several experiments with different application rates, Tim McCoy, Research Specialist at Virginia Tech Department of Entomology Dodson Urban Pest Management Laboratory found the solution to be very effective in killing all the bed bugs within 15 minutes.  A brief synopsis of each test follows:

1. Ten adult bed bugs (5 male, 5 female) in a 95 mm diameter glass Petri dishes were sprayed with 130 ul of the solution A+B (1:1 solution) from a distance of ~8 cm, from a pump atomizer.  All bugs were either immediately immobilized by the spray (6 bugs) or stopped walking within 3 minutes.  Bed bugs were considered “dead” if they did not respond to prodding with forceps. I was concerned that the volume of spray might be too large (i.e. simply drowning the bugs in a soapy solution) so I conducted a second test.

2. Five adult bed bugs (3 male, 2 female) in a 95 mm diameter glass Petri dishes were sprayed with 130 ul of solution A+B (1:1 solution) from a distance of ~8 cm.  After 2 minutes of exposure the bed bugs were removed from the dish and placed on filter paper so that excess solution could be wicked away.  The bed bugs recovered and were walking around erratically within 1 minute.  Several bugs were “stilt-walking” (legs extended and abdomen curled upwards) at this time.  All of these bugs were dead within 6 minutes after being removed from the treatment.

The slightly faster time to mortality might be caused by the wicking away of the solution which could have carried away the protective wax layer of the bed bug tracheal tubes, allowing residual surfactant/peroxide mixture to get directly into cells causing rapid death.  More experiments would be necessary to test this theory.

I was curious to see how the bed bugs reacted to the spray on a cloth surface.

3. Five adult bed bugs (3 male, 2 female) under a square of cloth (4 cm X 4 cm) in a 95 mm diameter glass Petri dishes were sprayed with 260 ul of solution A+B (1:1 solution) from a distance of ~8 cm.  I increased the volume to ensure that the bugs would be thoroughly wetted under the cloth.  At 4 minutes into test 1 bed bug was immobile and twitching and the other 4 were stilt-walking or moving quickly around the dish.  At 15 minutes all 5 bugs were moving slowly around the dish, clearly impaired but they appeared like they might recover.  I was required to leave the lab for the next 2 hours.  Upon returning I was surprised to find all 5 bed bugs dead.  The bugs were shriveled as though the solution caused desiccation…”